Malaria is still a global challenge with significant morbidity and mortality, especially
in the African, South-East Asian, and Latin American regions. Malaria diagnosis is
a crucial pillar in the control and elimination efforts, often accomplished by the
administration of mass-scale Rapid diagnostic tests (RDTs). The inherent limitations
of RDTs- insensitivity in scenarios of low transmission settings and deletion of one
of the target proteins- Histidine rich protein 2/3 (HRP-2/3) are evident from multiple
reports, thus necessitating the need to explore novel diagnostic tools/targets. The
present study used peptide microarray to screen potential epitopes from 13 antigenic
proteins (CSP, EXP1, LSA1, TRAP, AARP, AMA1, GLURP, MSP1, MSP2, MSP3, MSP4, P48/45,
HAP2) of P. falciparum. Three cyclic constrained immunoreactive peptides- C6 (EXP1), A8 (MSP2), B7 (GLURP)
were identified from 5458 cyclic constrained peptides (in duplicate) against P. falciparum-infected sera. Peptides (C6, A8, B7- cyclic constrained) and (G11, DSQ, NQN- corresponding
linear peptides) were fairly immunoreactive towards P. falciparum-infected sera in dot-blot assay. Using direct ELISA, cyclic constrained peptides
(C6 and B7) were found to be specific to P. falciparum-infected sera. A substantial number of samples were tested and the peptides successfully
differentiated the P. falciparum positive and negative samples with high confidence. In conclusion, the study identified
3 cyclic constrained immunoreactive peptides (C6, B7, and A8) from P. falciparum secretory/surface proteins and further validated for diagnostic potential of 2 peptides
(C6 and B7) with field-collected P. falciparum-infected sera samples.
Abbreviations:
CSP (circumsporozoite protein), EXP1 (exported protein 1), LSA1 (liver stage antigen 1), TRAP (thromobospondin-related anonymous protein), AARP (apical asparagine rich protein), GLURP (glutamate rich protein), MSP1 (merozoite surface protein 1), MSP2 (merozoite surface protein 2), MSP3 (merozoite surface protein 3), MSP4 (merozoite surface protein 4)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: June 09, 2022
Accepted:
June 6,
2022
Received in revised form:
June 4,
2022
Received:
October 31,
2021
Edited by Mr. M. FranklinIdentification
Copyright
© 2022 Elsevier Inc. All rights reserved.
